منابع مشابه
Comparison of Special Stains for Keratin with Routine Hematoxylin and Eosin Stain
BACKGROUND Keratins are the most abundant proteins and are characteristic findings in many epithelial pathologies, making it a diagnostically important marker, both histopathologically and immunohistochemically. Since, immunohistochemistry is an expensive diagnostic tool, special stains to detect the degree of keratinization could serve as a faster and economic option. The aim of the present st...
متن کاملRuthenium Red Staining of Chromatin in Epon Sections
For a long time, the ammoniated ruthenium oxychloride “ruthenium red” (today formulated [1, 2] as [(NH3)5 R u •O • Ru (NH3)4 O • Ru (NH3)5]6+ • Clg), has been used as a microscopic test for pectins [2, 3]. Several applications of this contrasting agent for electron microscopic detection of acid polysaccharides and proteoglycans have been stud ied [4-10]. Its use as a Schiff-like reagent was re...
متن کاملOrcein as a mucin stain for gastrointestinal tissue sections.
Although orcein has been in use for a long time as a stain for elastic fibres, it is only recently that it has been rediscovered for the demonstration of a variety of other substances. These include hepatitis B surface antigen (HBsAg),' 2 copper-associated protein,34 and more recently, intestinal sulphated mucins.35 Sipponen5 studied the normal distribution of orceinpositive mucin in the gastro...
متن کاملCorrelative light and electron microscopy of the frog adrenal gland cells using adjacent epon-embedded sections.
Correlative light and electron microscopy on the same cells of the adrenal gland of the frog, Rana nigromaculata, fixed in glutaraldehyde followed by osmium tetroxide, was done using the adjacent Epon embedded sections. Electron microscope observation revealed three different types of granule-filled secretory cells; the noradrenaline-storing cells (NA cells) filled with intensely dense and vary...
متن کاملAn allochrome periodic acid-methenamine silver stain for demonstrating mycobacteria in tissue sections.
Nyka9) employed the Gomori-Grocott's chromic acid-methenamine silver method for demonstrating tubercle bacilli in tissue sections after sublimate fixative with added hydrogen peroxide or periodic acid. He described that this procedure could demonstrate mycobacteria that had lost their acidfastness by the usual carbol fuchsin stain. Harada5) also employed the periodic acid-methenamine silver sta...
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ژورنال
عنوان ژورنال: Microscopy Today
سال: 1997
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s1551929500061423